PCR test to detect Xanthomonas axonopodis pv. dieffenbachiae
An immunocapture PCR test (IC-PCR) has been developed to detect Xanthomonas axonopodis pv. dieffenbachiae (EPPO Action List) in Anthurium plant tissues. This method combined the use of a monoclonal antibody specific of the genus Xanthomonas followed by PCR with specific primers. During this study, 3 sets of new specific primers were obtained. The IC-PCR was found to be more sensitive than the conventional PCR and indirect ELISA. The new primers could be used both for the diagnostic of X. axonopodis pv. dieffenbachiae in single PCR reactions or in multiplex reactions to differentiate between strains of the bacterium.
Sources
Khoodoo MHR, Sahin F, Jaufeerally-Fakim Y (2005) Sensitive detection of Xanthomonas axonopodis pv. dieffenbachiae on Anthurium andreanum by immunocapture-PCR (IC-PCR) using primers designed from sequence characterized amplified regions (SCAR) of the blight pathogen. European Journal of Plant Pathology, 112(4), 379-390.