Frankliniella fusca is a vector of Impatiens necrotic spot tospovirus.
So far, Impatiens necrotic spot tospovirus (INSV – EPPO A2 quarantine pest) was only known to be transmitted by Frankliniella occidentalis (Thysanoptera: Thripidae – EPPO A2 quarantine pest). Laboratory studies were done to determine whether INSV could also be transmitted by Frankliniella fusca. A population of virus-free F. fusca was reared on detached peanut leaves. Adults were confined on peanut leaves for oviposition, and then the peanut leaves (adults being removed) were examined daily for larval emergence. First instar larvae (;12 h old) were given an acquisition access period of 24 to 48 h on INSV-infected leaves of Emilia sonchifolia. Larvae were then transferred to healthy peanut leaves until adult emergence, and resulting adults were subsequently given a 48 h inoculation access period on healthy E. sonchifolia seedlings. Thrips were killed and plants maintained, awaiting symptom expression. 7 to 10 days after inoculation, plants developed symptoms (chlorotic spots, mosaic and mottling). The presence of INSV was confirmed by serological (ELISA) and molecular (PCR) tests. These experiments were repeated several times and demonstrated that F. fusca is a vector of INSV.
Sources
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Naidu, R.A.; Deom, C.M.; Sherwood, J.L. (2001) First report of Frankliniella fusca as a vector of Impatiens necrotic spot tospovirus.
Plant Disease, 85(1), p 1211.
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