Perspectives for better diagnosis of Curtobacterium flaccumfaciens pv. flaccumfaciens in bean seeds
Preliminary studies have been carried out in Italy on the improvement of detection methods for Curtobacterium flaccumfaciens pv. flaccumfaciens (EPPO A2 quarantine pest) in bean seeds.
1) In order to improve cultural methods, a semi-selective medium has been obtained for C. flaccumfaciens pv. flaccumfaciens
2) To develop better detection methods, work is being carried out on identification of stable metabolic traits. Preliminary studies showed that bacteriocins are produced by most strains and are of proteic nature. The final aim will be to find corresponding oligonucleotides to be used as primers for specific PCR detection of the bacterium in bean seeds.
Sources
Tegli, S.; Surico, G.; Esposito, A. (1997) Preliminary observations for the diagnosis of Curtobacterium flaccumfaciens pv. flaccumfaciens in bean seeds.
Proceedings of the 10th Congress of the Mediterranean Phytopathological Union, 1997-06-01/05, Montpellier (FR), 323-327.