Detection of Clavibacter michiganensis subsp. michiganensis in tomato seedlings
At the 1992 Annual Meeting of the American Phytopathological Society a technique was described to detect Clavibacter michiganensis subsp. michiganensis (EPPO A2 pest) in symptom-less tomato seedlings. Symptomless seedlings were surface sterilized, chopped in carbonate/bicarbonate buffer and the extract was used in an ELISA test utilizing monoclonal antibodies specific to the bacterium. With the technique, infected seedlings could be detected from commercial lots.
Sources
Gharbi, A.G.; Nameth, S.T. (1992) The use of monoclonal-base serodiagnostic assays for detection of Clavibacter michiganensis subsp. michiganensis in symptomless tomato seedlings.
Presentation at the 1992 APS/MPS Annual Meeting, Portland, OR, USA. Phytopathology 82, 1076.