Screening method for tomato lines tolerant to tomato yellow leaf curl geminivirus
Experiments were carried out in Israel to determine the accumulation of tomato yellow leaf curl geminivirus DNA in tomato plants tolerant and susceptible to tomato yellow leaf curl geminivirus (potential EPPO A2 pest).
It was found that in all tested tomato lines the viral DNA concentration peaked 12-15 days after inoculation and that at this time the viral DNA in the tolerant lines was 10-50% lower than in the susceptible ones. After this peak the viral DNA decreased up to 50% in tolerant and susceptible lines. A positive correlation was found between the viral DNA accumulation and the expression and severity of disease symptoms. The authors believe that this technique of measuring the viral DNA accumulation in tomato plants infected by TYLCV might be useful to screen fast and efficiently tomato lines in their susceptibility to TYLCV.
Sources
Rom, M.; Antignus, Y.; Gidoni, D.; Pilowsky, M.; Cohen, S. (1993) Accumulation of tomato yellow leaf curl virus DNA in tolerant and susceptible tomato lines.
Plant Disease 77, 253-257.