Medium for the cultivation of Xylella fastidiosa
A medium for the cultivation of strains of Xylella fastidiosa (EPPO A1 quarantine pest) isolated from grapevine has been developed in Georgia, US. The medium, called XF-26, supported the primary growth of the bacterium.
The formula for the medium is as follows:
Substance (g/L)
- K2HPO4 1,5
- (NH4)2HPO4 0,5
- MgSO4.7H2O 2,0
- Trisodium citrate 1,5
- Disodium succinate 1,5
- L-Alanine ; ; ; ;0,2
- L-Arginine 0,4
- L-Asparagine 0,4
- L-Cysteine 0,4
- Glycine 0,2
- L-Gutamine 2,0
- L-Histidine 0,4
- L-Isoleucine 0,2
- L-Leucine 0,2
- L-Lysine 0,2
- L-Methionine 0,2
- L-Phenylalanine 0,2
- L-Proline 0,2
- L-Serine 0,2
- L-Threonine 0,2
- L-Tryptophan 0,04
- L-Valine 0,2
- Phenol red 0,02
- Potato starch 0,2
- Difco agar 15,0
K2HPO4, (NH4)2HPO4, trisodium citrate, disodium succinate, potato starch and agar are dissolved in 800 ml of distilled water followed by adding the stock solutions. The concentrations of MgSO4.7H2O and phenol red were 10% and 0,2%, respectively. The concentration of the amino acids were 3,3% except those of glutamin and tryptophan which were 16,7 and 0,67%, respectively. All stock solutions were sterilized by autoclaving for 15 minutes. The final volume of the medium was brought up to one litre by adding distilled water and the pH was adjusted to 6,6-6,7 by adding NaOH. The XF-26 broth medium was prepared in the same way without the agar.
Sources
Chang, C.J.; Donaldson, R.C. (1993) Xylella fastidiosa: Cultivation in chemically defined medium.
Phytopathology 83, 192-194.